The Effect of Squalene Addition In-Vitro to Increase Asiaticoside Hairy Root Culture

The work on the potential of C. asiatica as a source of asiaticoside has been done
very little in Indonesia. However, it is very promising economically. By using the progress in
biotechnology in this case the use of the culture of hairy roots is expected to become a
prospective method for producing the secondary compound from C. asiatica. Culturing the
hairy roots is a method of producing secondary compound with many benefits compared to cell
culture or suspension. The objectives of this research were to obtain the best Squalene
concentration is important to obtain high production of triterpenoid secondary metabolite. The
research consist 4 treatments in Completely Randomized Design (CRD) with 6 replications.
As a treatment was concentration 0 ppm Squalene (control), 150 ppm, 300 ppm, 450 ppm. The
results showed that strains R.1000 of Agrobacterium rhizogenes and 300 ppm Squalene
concentration increased asiaticoside.